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DHARA is an online index of articles on Ayurveda published in research journals worldwide.
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Journal of Drug Research in Ayurvedic Sciences
2022
October - December
;
7
(4)
:229 -242
Detoxification of Datura metel L. seeds using Shodhana (purifying process) and estimation of scopolamine content
Ajay Kumar Meena (1)
,
Mohit Motiwale (1)
,
Poorna Venktaraman (2)
,
Kusuma Ganji (2)
,
Ravindra Singh (3)
,
Narayanam Srikanth (3)
,
Kartar Singh Dhiman (3)
,
Sadhna Chaturvedi (4)
,
Amit Kumar Dixit (5)
1. Department of Chemistry, Regional Ayurveda Research Institute, Gwalior, Madhya Pradesh, India 2. Department of Chemistry, Captain Srinivasa Murthy Central Ayurveda Research Institute, Chennai, Tamil Nadu, India 3. Central Council for Research in Ayurvedic Sciences, Ministry of Ayush, New Delhi, India 4. Department of Biotechnology, ITM University, Gwalior, Madhya Pradesh, India 5. Department of Biochemistry, Central Ayurveda Research Institute, Kolkata, West Bengal, India
Abstract
BACKGROUND: The seeds of Datura metel L. are classified as toxic in the classical texts of Ayurveda and hence need to be purified by the traditional method as mentioned before using for medications. METHODS: In this study, the seeds of Datura metel L. were detoxified by Shodhana, an Ayurvedic purifying process. The process of detoxification (Shodhana) was performed by soaking the seeds in gomutra (cow’s urine) and boiling them in godugdha (cow’s milk). The preliminary phytochemical and physicochemical analyses were performed simultaneously for the processed and unprocessed Datura metel L. seeds. The total ethanol and chloroform extracts of the processed and unprocessed Datura metel L. seed samples were used for chemical profiling using high-performance thin-layer chromatography, high-performance liquid chromatography (HPLC), liquid chromatography-mass spectrometer, and gas chromatography-mass spectrometer. Scopolamine content was quantified by HPLC in both processed and unprocessed seeds. RESULTS: Scopolamine was not detected in the chloroform extract. The ethanol extract found that the detoxification process has removed scopolamine content in the processed Datura metel L. seed. An attempt has been made to identify the chemical constituents responsible for the toxicity by measuring the level of major chemical constituents before and after the detoxification of seeds. CONCLUSION: This study reveals that the process of Shodhana was effective by reducing the content of scopolamine, and hence it was mandatory and supports its use in medications. Keywords: Datura metel, detoxification, HPLC, scopolamine, Shodhana
DHARA ID:
D060872
Link To Full Paper
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