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DHARA is an online index of articles on Ayurveda published in research journals worldwide.
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Journal of Drug Research in Ayurvedic Sciences
2018
January- March
;
3
(1)
:9-14
Micropropagation of Dhumrapatra (Aristolochia bracteolata Lam.): An Important Medicinal Plant of Ayurveda
Archana G Mhase (1)
,
Ritu Sinha (2)
,
Arun M Gurav (3)
,
Gajendra Rao (3)
,
Anupam K Mangal (4)
,
Narayanam Srikanth (5)
1. Herbarium Assistant, Regional Ayurveda Institute for Fundamental Research, Pune and Central Council for Research in Ayurvedic Sciences, Pune Maharashtra, India 2. Lab. Technician,Regional Ayurveda Institute for Fundamental Research, Pune and Central Council for Research in Ayurvedic Sciences, Pune Maharashtra, India 3. Research Officer (Botany), Regional Ayurveda Institute for Fundamental Research, Pune and Central Council for Research in Ayurvedic Sciences, Pune Maharashtra, India 4. Assistant Director (Pharmacognosy), Central Council for Research in Ayurvedic Sciences, New Delhi, India 5. Deputy Director General, Central Council for Research in Ayurvedic Sciences, New Delhi, India
Abstract
Aim: Dhumrapatra is an important medicinal plant used in Indian system of medicine. Due to overexploitation and unsystematic collection, it is becoming rare and endangered in some parts of India. To meet the demand of the authentic drug and propagate the plant on a large scale, an in vitro propagation technique has been developed for Dhumrapatra. Materials and methods: The collected plant was identified with the help of floras. Murashige and Skoog basal (MS) medium was prepared as per the standard protocol. Surface sterilization of explants was done with the help of disinfectant and 0.1% solution of HgCl2. Media were autoclaved at 121°C under 15 lb/inch2 pressure for 15 minutes. Cultures were incubated at 22°C ± 2°C and 8 hours photoperiod with light intensity of 3000 lux. Observations were recorded after a 4-week period. Results: Best establishment of shoots (20 nos.) was found in MS media augmented with 0.5 mg/L kinetin (Kn). Maximum roots (2–3 nos) produced in MS were fortified with 3 mg/L indolebutyric acid (IBA). Maximum number of shoots and roots were achieved on nodal explants inoculated on MS + Kn (0.5 mg/L) + 0.1% polyvinylpyrrolidone (PVP). Conclusion: The in vitro propagation protocol developed for Dhumrapatra would be beneficial for rapid multiplication and conservation of important medicinal plant. Keywords: Conservation, Dhumrapatra, Hormones, In vitro, Regeneration.
DHARA ID:
D060729
Link To Full Paper
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