Search Details

Home | About | Quick Start | FAQ

Beta Version 0.1

DHARA is an online index of articles on Ayurveda published in research journals worldwide.

Users Online:

78

Unfiltered Classified Search

Enter Keyword Below :


Advanced


Journal of Drug Research in Ayurvedic Sciences 2017 October - December ; 2 (4) :267-273
Conservation of Manjishtha—Rubia cordifolia L. through Nodal Culture
Arun M Gurav (1), Arun M Gurav (1), Ritu Sinha (2), Ritu Sinha (2), Archana G Mhase (3), Archana G Mhase (3), Gajendra Rao (4), Gajendra Rao (4), Anupam K Mangal (5), Anupam K Mangal (5), Narayanam Srikanth (6), Narayanam Srikanth (6)
1. Research Officer (Botany),Regional Ayurveda Institute for Fundamental Research Pune, Maharashtra, India 2. Lab Technician, Regional Ayurveda Institute for Fundamental Research Pune, Maharashtra, India 3. Herbarium Assistant, Regional Ayurveda Institute for Fundamental Research Pune, Maharashtra, India 4. Research Officer (Botany),Regional Ayurveda Institute for Fundamental Research Pune, Maharashtra, India 5. Assistant Director (Pharmacognosy), Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, New Delhi, India 6. Deputy Director General, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, New Delhi, India
Abstract
Aim: Manjishtha—Rubia cordifolia L. (family: Rubiaceae) is an important medicinal plant and used in various Ayurvedic formulations. Plant parts like roots, stems, leaves and fruits are being used to treat various respiratory and skin diseases. Manjistha is excessively collected from natural habitat and becoming rare and vulnerable in different parts of country. Therefore, it is decided to develop a systematic in vitro protocol for rapid multiplication of the plant. Materials and methods: Nodal segments collected from healthy, desease free plant were used as explants. Pretreated and surface sterilized nodal segments were implanted on to MS basal medium as well as MS fortified with different concentrations of plant growth regulators viz., BAP, TDZ, Kn, NAA, IAA, IBA singly or in combinations. Then, the cultures were incubated at 22°C ± 2°C for 8 hours photoperiod with light intensity of 3000 lux. Results: Maximum number of shoots (20–25) developed from the nodal segments inoculated on MS + TDZ (0.5 mg/l) + 0.1% PVP liquid medium. The best rooting (2–3 roots) were developed in MS + IBA (2 mg/l) in 8 to 14 days. Conclusion: The in vitro protocol developed would be beneficial to multiply the plants of R. cordifolia on large scale within the short period with low cost and to conserve the plant. Keywords: Conservation, Growth regulators, In vitro propagation, Micropropagation, Node.
DHARA ID: D060721
Link To Full Paper

Copyright \| Disclaimer \| Feedback \| Updates \| Contact
Developed and maintained by AVP Research Foundation (Formerly AVT Institute for Advanced Research), 136/137, Trichy Road, Ramanathapuram, Coimbatore - 641045, Tamil Nadu, India
Funded by Central Council for Research in Ayurvedic Sciences, Dept. of AYUSH, Ministry of Health and Family Welfare, Government of India, New Delhi