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Journal of separation science 2021 November ; 44 (22) :4064-4081 [Epub ahead of Print]
Phytometabolite profiling of Coronil, a herbal medicine for COVID-19, its identification by mass-spectroscopy and quality validation on liquid chromatographic platforms
Acharya Balkrishna (1), Acharya Balkrishna (1), Meenu Tomer (1), Meenu Tomer (1), Sudeep Verma (1), Sudeep Verma (1), Monali Joshi (2), Monali Joshi (2), Priyanka Sharma (1), Priyanka Sharma (1), Jyotish Srivastava (2), Jyotish Srivastava (2), Anurag Varshney (2), Anurag Varshney (2), Acharya Balkrishna (3), Acharya Balkrishna (3), Anurag Varshney (3), Anurag Varshney (3), Anurag Varshney (4), Anurag Varshney (4)
1. Drug Discovery and Development Division, Patanjali Research Institute, NH-58, Haridwar, Uttarakhand, 249405, India 2. Drug Discovery and Development Division, Patanjali Research Institute, NH-58, Haridwar, Uttarakhand, 249405, India 3. Department of Allied and Applied Sciences, University of Patanjali, Patanjali Yogpeeth, NH-58, Haridwar, Uttarakhand, 249405, India. 4. Special Centre for Systems Medicine, Jawaharlal Nehru University, New Delhi, 110067, India
Abstract
Coronil is a tri-herbal medicine consisting of immunomodulatory herbs, Withania somnifera, Tinospora cordifolia, and Ocimum sanctum. The formulation has been developed specifically as the supporting measure for COVID-19. Current investigation is aimed to identify the phytoconstituents in Coronil utilizing ultra-performance liquid chromatography-mass spectrometry coupled with quadrapole time of flight and to establish its quality standardization using high-performance liquid chromatography and high performance thin layer chromatography. Out of 52 identified compounds, cordifolioside A, magnoflorine, rosmarinic acid, palmatine, withanoside IV, withanoside V, withanone, betulinic acid, and ursolic acid were quantified in 15 different batches of Coronil on validated high-performance liquid chromatography method. Similarly, withanoside IV, withaferin A, magnoflorine, palmatine, rosmarinic acid, and ursolic acid were analyzed on high performance thin layer chromatography. Methods were validated as per the International Council for Harmonization guidelines. These methods were specific, reproducible, accurate, precise, linear (r2 > 0.99), and percent recoveries were within the prescribed limits. The content uniformity of Coronil was ascertained using Fourier transform infrared spectroscopy. Results indicated that, validated methods were fit for their intended use and the analytical quality of Coronil was consistent across the batches. Taken together, these developed methods could drive the analytical quality control of herbal medicines such as Coronil, and other formulations containing similar chemical profiles. Keywords: Coronil; ayurveda; liquid chromatography; mass spectrometry; validation.
DHARA ID: D058232 Pubmed ID: 34510772
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