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DHARA is an online index of articles on Ayurveda published in research journals worldwide.
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Nutrition and Cancer
2016
May
:0
Parkia javanica Extract Induces Apoptosis in S-180 Cells via the Intrinsic Pathway of Apoptosis.
Patra Kartick K (1)
,
Jana Samarjit S (1)
,
Sarkar Arnab A (1)
,
Karmakar Subrata S (1)
,
Jana Jagannath J (2)
,
Gupta Mradu M (3)
,
Mukherjee Gopeswar G (4)
,
De Utpal Chandra UC (5)
,
Mandal Deba Prasad DP (1)
,
Bhattacharjee Shamee S (1)
1. a Department of Zoology , West Bengal State University , Kolkata , India. 2. b Department of Biophysics , Bose Institute , Kolkata , India. 3. c Department of Dravyaguna (Medicinal Plant Pharmacology) , Institute of Post Graduate Ayurvedic Education & Research , Kolkata , India. 4. d Department of Pathology , Barasat District Hospital , Kolkata , India. 5. e Department of Chemistry , Tripura University , Suryamaninagar, Agartala , India.
Abstract
Parkia javanica is a leguminous tree, various parts of which are used as food and folklore medicine by the ethnic groups of northeastern India. The present study investigates the in vitro and in vivo anticancer effect of aqueous methanol extract of P. javanica fruit (PJE). HPLC analysis was done to establish the fingerprint chromatogram of PJE and its in vitro radical scavenging activity was measured. PJE caused significant cytotoxicity in sarcoma-180 (S-180), A549, AGS, and MDA-MB435S cancer cells in vitro. Exploration of the mechanistic details in S-180 cells suggested that the reduced cell viability was mediated by induction of apoptosis. Increased expression of proapoptotic proteins such as p53, p21, Bax/Bcl2, cytochrome c (Cyt c), caspase 9, and cleaved poly(ADP-ribose) polymerase, and decrease in proliferative and antiapoptotic markers (Ki-67, Proliferating Cell Nuclear Antigen [PCNA], Bcl-2) validated the anticancer effect of PJE. A decline in the relative fluorescence emission upon staining S-180 cells with Rhodamine 123 (Rh 123), enhanced expression of cytosolic Cyt c and mitochondrial Bax, and inhibition of apoptosis in the presence of caspase-9 inhibitor in PJE-treated cells indicated intrinsic pathway of apoptosis. Liver function test and hepatic antioxidant enzymes demonstrated non-toxicity of PJE. Finally, the detection of PJE in sera by HPLC confirmed its bioavailability.
DHARA ID:
D055830
Pubmed ID:
27144503
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