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The Plant journal : for cell and molecular biology 2011 Jan ; (2) :0
OsPUB15, an E3 ubiquitin ligase, functions to reduce cellular oxidative stress during seedling establishment.
Park JJ,   Yi J,   Yoon J,   Cho LH,   Ping J,   Jeong HJ,   Cho SK,   Kim WT,   An G  

Abstract
The plant U-box (PUB) protein functions as an E3 ligase to poly-ubiquitinate a target protein for its degradation or post-translational modification. Here, we report functional roles for OsPUB15, which encodes a cytosolic U-box protein in the class-II PUB family. Self-ubiquitination assays showed that bacterially expressed MBP-OsPUB15 protein has E3 ubiquitin ligase activity. A T-DNA insertional mutation in OsPUB15 caused severe growth retardation and a seedling-lethal phenotype. Mutant seeds did not produce primary roots, and their shoot development was significantly delayed. Transgenic plants expressing the OsPUB15 antisense transcript phenocopied these mutant characters. The abnormal phenotypes were partially rescued by two antioxidants, catechin and ascorbic acid. Germinating seeds in the dark also recovered the rootless defect. Levels of H2O2 and oxidized proteins were higher in the knock-out mutant compared with the wild type. OsPUB15 transcript levels were increased upon H2O2, salt and drought stresses; plants overexpressing the gene grew better than the wild type under high salinity. These results indicate that PUB15 is a regulator that reduces reactive oxygen species (ROS) stress and cell death.© 2010 The Authors. The Plant Journal © 2010 Blackwell Publishing Ltd.

DHARA ID: D052773 Pubmed ID: 21223385


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