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Journal of Ethnopharmacology 2011 Jan ; (2) :0
Anti-inflammatory action of methanol extract of Carthamus tinctorius involves in heme oxygenase-1 induction.
Jun MS,   Ha YM,   Kim HS,   Jang HJ,   Kim YM,   Lee YS,   Kim HJ,   Seo HG,   Lee JH,   Lee SH,   Chang KC  

Abstract
The methanol extracts of Carthamus tinctorius (MEC) have long been used in traditional medicine as anti-inflammatory agent, however, the molecular mechanism by which MEC shows anti-inflammatory action is not investigated.Induction of heme oxygenase-1 (HO-1) by many medicinal herbs has been reported excellent anti-inflammatory action. Thus, the aim of the study is to explore whether anti-inflammatory action of MEC is related with HO-1 induction in RAW 264.7 cells.The present study was designed to investigate as to MEC induces HO-1 expression so that it reduces inflammation by suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in cells activated with lipopolysaccharide (LPS).Expression of HO-1 protein by MEC in macrophages was increased in a concentration- and time-dependent manner. Treatment with MEC significantly inhibited upregulation of both iNOS and COX-2 in LPS-activated macrophages and consequently reduced production of NO and PGE(2), respectively. The reduced expression of iNOS and COX-2 by MEC was reversed by siHO-1 RNA transfection. In addition, NF-E2-related factor (Nrf2) was translocated from cytosol to nucleus by MEC. The binding of NF-?B as well as NF-?B luciferase activity was also significantly diminished by MEC. Finally, tumor necrosis factor (TNF)-a-mediated VCAM-1 expression in endothelial cell was significantly inhibited by MEC.The present results show that MEC induces HO-1 expression via Nrf2 translocation and inhibits NF-?B activity, which may be responsible for anti-inflammatory action. Therefore, we propose that anti-inflammatory action of MEC involves at least HO-1 induction.Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.

DHARA ID: D051206 Pubmed ID: 20969944


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