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DHARA is an online index of articles on Ayurveda published in research journals worldwide.
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Mycopathologia
2011
May
;
(5)
:0
Real-time PCR assay based on topoisomerase-II gene for detection of Fusarium udum.
Yadav MK
,
Babu BK
,
Saxena AK
,
Singh BP
,
Singh K
,
Arora DK
Abstract
Fusarium wilt is an important soilborne disease of pigeonpea, caused by Fusarium udum. In this study, we have designed a real-time PCR assay for the detection of Fusarium udum from infected pigeonpea plants. Based on Topoisomerase-II gene sequence data from Fusarium udum and other related Fusarium species, a pair of primer was designed. The species-specific primers were tested in real-time PCR SYBR green assay. No increasing fluorescence signals exceeding the baseline threshold was observed with tested microbes, except Fusarium udum DNA. A single dissociation peak of increased fluorescence was obtained for the specific primers at melting temperature of 81.25°C. The real-time PCR showed a lowest detection of 0.1 pg genomic DNA. The assay was more sensitive, accurate and less time consuming for detection of Fusarium udum in infected plants root.
DHARA ID:
D036351
Pubmed ID:
21104322
Access to Full Paper Not Available
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