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DHARA is an online index of articles on Ayurveda published in research journals worldwide.
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The Plant journal : for cell and molecular biology
2000
Jun
;
(6)
:0
T-DNA insertional mutagenesis for functional genomics in rice.
Jeon JS
,
Lee S
,
Jung KH
,
Jun SH
,
Jeong DH
,
Lee J
,
Kim C
,
Jang S
,
Yang K
,
Nam J
,
An K
,
Han MJ
,
Sung RJ
,
Choi HS
,
Yu JH
,
Choi JH
,
Cho SY
,
Cha SS
,
Kim SI
,
An G
Abstract
We have produced 22 090 primary transgenic rice plants that carry a T-DNA insertion, which has resulted in 18 358 fertile lines. Genomic DNA gel-blot and PCR analyses have shown that approximately 65% of the population contains more than one copy of the inserted T-DNA. Hygromycin resistance tests revealed that transgenic plants contain an average of 1.4 loci of T-DNA inserts. Therefore, it can be estimated that approximately 25 700 taggings have been generated. The binary vector used in the insertion contained the promoterless beta-glucuronidase (GUS) reporter gene with an intron and multiple splicing donors and acceptors immediately next to the right border. Therefore, this gene trap vector is able to detect a gene fusion between GUS and an endogenous gene, which is tagged by T-DNA. Histochemical GUS assays were carried out in the leaves and roots from 5353 lines, mature flowers from 7026 lines, and developing seeds from 1948 lines. The data revealed that 1.6-2.1% of tested organs were GUS-positive in the tested organs, and that their GUS expression patterns were organ- or tissue-specific or ubiquitous in all parts of the plant. The large population of T-DNA-tagged lines will be useful for identifying insertional mutants in various genes and for discovering new genes in rice.
DHARA ID:
D026830
Pubmed ID:
10886776
Access to Full Paper Not Available
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