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DHARA is an online index of articles on Ayurveda published in research journals worldwide.
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Nature
2005
Sep
;
(7059)
:0
GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin.
Ueguchi-Tanaka M
,
Ashikari M
,
Nakajima M
,
Itoh H
,
Katoh E
,
Kobayashi M
,
Chow TY
,
Hsing YI
,
Kitano H
,
Yamaguchi I
,
Matsuoka M
Abstract
Gibberellins (GAs) are phytohormones that are essential for many developmental processes in plants. It has been postulated that plants have both membrane-bound and soluble GA receptors; however, no GA receptors have yet been identified. Here we report the isolation and characterization of a new GA-insensitive dwarf mutant of rice, gid1. The GID1 gene encodes an unknown protein with similarity to the hormone-sensitive lipases, and we observed preferential localization of a GID1-green fluorescent protein (GFP) signal in nuclei. Recombinant glutathione S-transferase (GST)-GID1 had a high affinity only for biologically active GAs, whereas mutated GST-GID1 corresponding to three gid1 alleles had no GA-binding affinity. The dissociation constant for GA4 was estimated to be around 10(-7) M, enough to account for the GA dependency of shoot elongation. Moreover, GID1 bound to SLR1, a rice DELLA protein, in a GA-dependent manner in yeast cells. GID1 overexpression resulted in a GA-hypersensitive phenotype. Together, our results indicate that GID1 is a soluble receptor mediating GA signalling in rice.
DHARA ID:
D023948
Pubmed ID:
16193045
Access to Full Paper Not Available
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