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DHARA is an online index of articles on Ayurveda published in research journals worldwide.
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Bioscience, biotechnology, and biochemistry
2008
Dec
;
(12)
:0
Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system.
Ko KW
,
Lin F
,
Katsumata T
,
Sugai Y
,
Miyazaki S
,
Kawaide H
,
Okada K
,
Nojiri H
,
Yamane H
Abstract
Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis. We found that microsomes from the methylotropic yeast Pichia pastoris expressing both OsKO2 and a fungal cytochrome P450 monooxygenase (P450) reductase converted ent-kaurene to ent-kaurenoic acid. This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice.
DHARA ID:
D018312
Pubmed ID:
19060383
Access to Full Paper Not Available
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