Home
|
About
|
Quick Start
|
FAQ
Beta Version 0.1
DHARA is an online index of articles on Ayurveda published in research journals worldwide.
Users Online:
235
Unfiltered
Classified Search
Enter Keyword Below :
Advanced
PloS One
2009
;
(8)
:0
Protein-protein interactions of tandem affinity purified protein kinases from rice.
Rohila JS
,
Chen M
,
Chen S
,
Chen J
,
Cerny RL
,
Dardick C
,
Canlas P
,
Fujii H
,
Gribskov M
,
Kanrar S
,
Knoflicek L
,
Stevenson B
,
Xie M
,
Xu X
,
Zheng X
,
Zhu JK
,
Ronald P
,
Fromm ME
Abstract
Eighty-eight rice (Oryza sativa) cDNAs encoding rice leaf expressed protein kinases (PKs) were fused to a Tandem Affinity Purification tag (TAP-tag) and expressed in transgenic rice plants. The TAP-tagged PKs and interacting proteins were purified from the T1 progeny of the transgenic rice plants and identified by tandem mass spectrometry. Forty-five TAP-tagged PKs were recovered in this study and thirteen of these were found to interact with other rice proteins with a high probability score. In vivo phosphorylated sites were found for three of the PKs. A comparison of the TAP-tagged data from a combined analysis of 129 TAP-tagged rice protein kinases with a concurrent screen using yeast two hybrid methods identified an evolutionarily new rice protein that interacts with the well conserved cell division cycle 2 (CDC2) protein complex.
DHARA ID:
D018048
Pubmed ID:
19690613
Access to Full Paper Not Available
Copyright
|
Disclaimer
|
Feedback
|
Updates
|
Contact
Developed and maintained by AVP Research Foundation (Formerly AVT Institute for Advanced Research), 136/137, Trichy Road, Ramanathapuram, Coimbatore - 641045, Tamil Nadu, India
Funded by Central Council for Research in Ayurvedic Sciences, Dept. of AYUSH, Ministry of Health and Family Welfare, Government of India, New Delhi