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DHARA is an online index of articles on Ayurveda published in research journals worldwide.
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Bioscience, biotechnology, and biochemistry
2010
;
(2)
:0
Gene cloning, bacterial expression, and purification of a novel rice (Oryza sativa L.) ubiquitin-related protein, RURM1.
Tsukiyama T
,
Lee J
,
Okumoto Y
,
Teraishi M
,
Tanisaka T
,
Inouye K
Abstract
A rice ubiquitin-related modifier-1 (Rurm1) gene was cloned and transformed in Escherichia coli. We successfully expressed the RURM1 protein as a glutathione S-transferase (GST)-fusion protein by cultivating the E. coli cells at 16 degrees C for 16 h. After cleavage of GST, we obtained a single protein of 12 kDa. This protein was identified as the RURM1 protein by western blot analysis.
DHARA ID:
D017191
Pubmed ID:
20139593
Access to Full Paper Not Available
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