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Phytochemical analysis : PCA
2015
Dec
:0
Analysis of phytochemical variations in dioecious Tinospora cordifolia stems using HPLC/QTOF MS/MS and UPLC/QqQLIT -MS/MS.
Bajpai Vikas V Academy of Scientific and Innovative Research, New Delhi, 110001, India. (1)
,
Singh Awantika A Academy of Scientific and Innovative Research, New Delhi, 110001, India. (1)
,
Chandra Preeti P Academy of Scientific and Innovative Research, New Delhi, 110001, India. (1)
,
Negi M P S MP (2)
,
Kumar Nikhil N (3)
,
Kumar Brijesh B Academy of Scientific and Innovative Research, New Delhi, 110001, India. (1)
1. Sophisticated Analytical Instrument Facility Division, CSIR-Central Drug Research Institute, Lucknow, 226031, India. 2. Biometry and Statistics Division, CSIR-Central Drug Research Institute, Lucknow, 226031, India. 3. Betelvine Laboratory, CSIR-National Botanical Research Institute, Lucknow, 226001, India.
Abstract
The stem of dioecious Tinospora cordifolia (Menispermaceae) is a commonly used traditional Ayurvedic medicine in India having several therapeutic properties.To develop and validate LC-MS methods for the identification and simultaneous quantitation of various secondary metabolites and to study metabolomic variations in the stem of male and female plants.Ethanolic extract of stems were analysed by HPLC/ESI-QTOF-MS/MS for rapid screening of bioactive phytochemicals. High resolution MS and MS/MS in positive ESI mode were used for structural investigation of secondary metabolites. An UPLC/ESI-QqQLIT -MS/MS method in MRM mode was developed and validated for the simultaneous quantitation of five bioactive alkaloids.Identification and characterisation of 36 metabolites including alkaloids, sesquiterpenes and phytoecdysteroids were performed using LC-MS and MS/MS techniques. The bioactive alkaloids such as jatrorrhizine, magnoflorine, isocorydine, palmatine and tetrahydropalmatine were successfully quantified in male and female plants. The mean abundances of magnoflorine jatrorrhizine, and oblongine were significantly (P?
DHARA ID:
D055708
Pubmed ID:
26627195
Access to Full Paper Not Available
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