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Journal of Ethnopharmacology 2015 Sep :0
Extract of Woodfordia fruticosa flowers ameliorates hyperglycaemia and oxidative stress, and improves ß-cell function in streptozotocin-nicotinamide induced diabetic rat.

Abstract
The art of Ayurveda and the traditional healing system in India have reflected the ethnomedicinal importance of the plant Woodfordia fruticosa Kurtz, which demonstrates its vast usage in the Ayurvedic preparations as well as in the management of diabetes by the traditional healers of Beed district in Maharashtra.The study aimed to ascertain the antidiabetic potential of Woodfordia fruticosa flower methanolic extract (WF) on Streptozotocin (STZ)-nicotinamide-induced diabetic rat model.Diabetes was induced in Sprague Dawley (SD) male rats by STZ-nicotinamide and the diabetic rats were treated with three different doses of WF (100, 200 and 400mg/kg body weight) and glibenclamide as a positive control. Biochemical parameters such as blood glucose, serum insulin and C-peptide levels were measured with oxidative stress markers. Moreover, histology of liver and pancreas were carried out to evaluate glycogen content and ß-cells structures, thereafter immunohistochemistry and western blot analysis were carried out on kidney and pancreas tissues to determine renal Bcl-2, pancreatic insulin and glucose transporter (GLUT-2, 4) proteins expression in all the experimental groups.The acute toxicity study confirmed the non-toxic nature of all the three doses of WF and further study models revealed the anti-hyperglycemic effects of WF by upregulating serum insulin and C-peptide levels in the diabetic rats. Similarly, WF shown to ameliorate oxidative stress by downregulating LPO levels and augmenting the antioxidant enzyme (ABTS). Furthermore, histopathological analysis demonstrate recovery in the structural degeneration of ß-cells mass of pancreas tissue with increase in the liver glycogen content of the diabetic rats. The protective nature of the extract was further confirmed by the immunohistochemical study result which displayed upregulation in the insulin and renal Bcl-2 expression, the anti apoptosis protein. Moreover, the western blot result shown slight alteration in the GLUT-2 and GLUT-4 protein expression with the highest dosage of WFc treatment, this might have stimulated glucose uptake in the pancreas and played an important role in attenuating the blood glucose levels.The data of the present study revealed the potential of WF in the management of diabetes and its related complications, thus warrants further investigation on its major compounds with in depth mechanistic studies at molecular level.Copyright © 2015. Published by Elsevier Ireland Ltd.

DHARA ID: D055661 Pubmed ID: 26342523


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