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DHARA is an online index of articles on Ayurveda published in research journals worldwide.
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Phytotherapy Research : PTR
2008
Nov
;
(11)
:0
Nrf2-mediated induction of detoxifying enzymes by alantolactone present in Inula helenium.
Seo JY
,
Lim SS
,
Kim JR
,
Lim JS
,
Ha YR
,
Lee IA
,
Kim EJ
,
Park JH
,
Kim JS
Abstract
Our previous study showed that a methanol extract of Inula helenium had the potential to induce detoxifying enzymes such as quinone reductase (QR) and glutathione S-transferase (GST) activity. In this study the methanol extract was further fractionated using silica gel chromatography and vacuum liquid chromatography, to yield pure compounds alantolactone and isoalantolactone as QR inducers. Alantolactone caused a dose-dependent induction of antioxidant enzymes including QR, GST, gamma-glutamylcysteine synthase, glutathione reductase, and heme oxygenase 1 in hepa1c1c7 mouse hepatoma cells. The compound increased the luciferase activity of HepG2-C8 cells, transfectants carrying antioxidant response element (ARE)-luciferase gene, in a dose-dependent manner, suggesting ARE-mediated transcriptional activation of antioxidant enzymes. Alantolactone also stimulated the nuclear accumulation of Nrf2 that was inhibited by phosphatidylinositol 3-kinase (PI3K) inhibitors. In conclusion, alantolactone appears to induce detoxifying enzymes via activation of PI3K and JNK signaling pathways, leading to translocation of Nrf2, and subsequent interaction between Nrf2 and ARE in the encoding genes.
DHARA ID:
D047099
Pubmed ID:
18702092
Access to Full Paper Not Available
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