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DHARA is an online index of articles on Ayurveda published in research journals worldwide.
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Bioscience, biotechnology, and biochemistry
2007
Feb
;
(2)
:0
Highly sensitive quantitative analysis of nicotianamine using LC/ESI-TOF-MS with an internal standard.
Wada Y
,
Yamaguchi I
,
Takahashi M
,
Nakanishi H
,
Mori S
,
Nishizawa NK
Abstract
A highly sensitive quantitative method for analyzing nicotianamine (NA) by liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) is reported. Fluorenylmethoxycarbonylation of nicotianamine reduced its polarity and enabled its retention in a reversed-phase column. The adoption of N(epsilon)-nicotyllysine (NL) as an internal standard ensured reliable quantification by giving a linear calibration curve drawn between the NA/NL molar ratios of standard solutions injected and the NA/NL area ratios in mass chromatograms. The high sensitivity of this analytical method allowed us to measure the amount of NA. This analytical method has applications to all research concerning NA.
DHARA ID:
D020914
Pubmed ID:
17284859
Access to Full Paper Not Available
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